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You are here: Home > Products > Physical > Spectrophotometer > Fluorescence Spectrophotometer > UFluo ONE I / UFluo ONE I...Products

UFluo ONE I / UFluo ONE II / UFluo ONE III

UFluo ONE Fluorometer

UFluo ONE Fluorometer

Fast and sensitive quantification of DNA, RNA, and protein

UFluo One Fluorometer is a benchtop fluorometer designed for highly accurate quantification of DNA, RNA, micro RNA, and protein. It is a dual-channel fluorometer and is ideal for the measurement of scarce and difficult-processing sample. The fluorometer has a 7 inch touch screen and intuitive user interface, making it easy to select and run your desired assay with results displayed in just a few seconds.

 

Key features

• High sensitivity —more sensitive than UV absorbance–based quantification

• Ideal for precious samples —uses as little as 0.5 μL of sample

• Flexible data management —store up to 100,000 samples internally or export results to the USB drive, or computer

• Portable with small footprint —small enough to fit in any lab

• Open reagent system — users can choose reagents freely, sample tube can be replaced, can order 0.5ml/0.2ml PCR tube

• Intuitive touchscreen —quickly get to data generation

• Accuracy and speed —accurately quantifies DNA, RNA, or protein in less than 3 seconds per sample

 

Model

 

Channel

 

Excitation

wavelength

Emission

wavelength

UFluo ONE I

 

Blue

470nm

525nm

Red

625nm

690nm

UFluo ONE II

Blue

470nm

525nm

Green

525nm

620nm

UFluo ONE III

 

Blue

470nm

525nm

UV

365nm

460nm

 

Common reagents and application

Excitation wavelength

Commonly used fluorescent reagents

Applications

 

365

Hoechst33258, 4-MU,EnZCheK Caspase

Plant GUS report gene detection,

cell apoptosis detection

 

 

470

 

PicoGreen®, oligreen,RiboGreen®, GFP, Protein, Fluorescein, Quant-iT™

DsDNA,ssDNA quantitative,  green fluorescent protein (GFP), fluorescent detection, protein quantitative

 

 

525

 

 

Rhodamine, Cy3, RFP Vybrant Cytotoxicity

Rhodamine detection, Cy-3 fluorescence labeling detection, red fluorescent protein (RFP) gene detection, cell activity toxicity detection

 

625

 

Cy5, Quant-iT RNA

 

Cy-5  fluorescence labeling detection, RNA quantitative

 

Specifications

Physical characteristics

Instrument type

Benchtop fluorometer

Dimensions(W*D*H)

(26cm× 16 cm× 6 cm); rectangular shape

 

Weight

1.0 kg

 

Operating Power

DC 9V 1.5A

Technical

Dynamic range

5 orders of magnitude

 

Detectors

Photodiodes

 

Repeatability

<1.5%

 

Stability

<1.5%

 

Linearity

R2>0.995

 

Sensitivity

dsDNA: 0.5ng/ml

 

Tube type

0.2mL/0.5mL

Hardware

Display

7-inch capacitive touchscreen with high resolution color display

 

Output ports

3× USB ports

 

Networking capability

Connection via the PC USB port using an Ethernet cable

 

Power supply

AC adaptor with power cords

USB drive

Capacity

16 Gigabyte